Edward J. (EJ) Crane III
R. C. Seaver Biology Building, Room 021
175 W Sixth Street
Claremont, CA 91711
Ph.D. The Johns Hopkins University,
B.A. St. Cloud State University, St. Cloud, MN
Postdoctoral Fellow, The Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC
Assistant Professor of Chemistry, Salisbury University, Salisbury, MD
Visiting Professor of Biochemistry, The
Tokyo Agricultural University, Tokyo, Japan
Assistant and Associate Professor of Chemistry, Pomona College
Chair of Chemistry, Pomona College
Biology 169, Microbial Ecology
Chemistry 115, Biochemistry
Chemistry 180, Advanced Biochemistry
We use techniques in biochemistry, electrochemistry and microbial ecology to characterize the biochemistry of microbes that inhabit a wide range of geologic features (including subsurface hydrothermal and petroleum systems, hydrothermal mud volcanoes, hydrothermal vents, acidic hot springs and aquatic and marine sediments). We are specifically interested in the systems used by these organisms in electron-transfer reactions. These reactions can provide the driving force for the life of the organism, be used to maintain an internal “balance” of oxidizing and reducing compounds, or protect against oxidative and other stresses. Many geologically important reactions are controlled by this biochemistry, and we are specifically interested in sulfur-based respiration and the role that microbes play in the sulfur cycle and in the formation and breakdown of sulfur-containing compounds and minerals.
Herwald, S., Liu, A., Zhu, B., Sea, K., Lopez, K., Sazinsky, M., and Crane III, E.J. Characterization of the structure and substrate specificity of the pyrococcal CoADR/Psr: Implications for S0-based respiration and a sulfur-dependent antioxidant system in Pyrococcus. Biochemistry, 52:2764-2773 (2013).
Prokopenko, M.G., Hirst M.B., De Brabandere, L., Berelson, W.M, D.J. Lawrence, D.J., Granger, J., Chang, B.X., S. Dawson, S., Crane, E.J. III, Chong , L., Thamdrup, B., D.M. Sigman, D.M. Thioploca/anammox consortia drive nitrogen losses in anoxic sediments. Nature, in press (2013).
Lancaster, K., Farver, O., Wherland, S., Crane, E.J. III, Richards, J.H., Israel Pecht, I., and Gray, H.B., Electron Transfer Reactivity of Type Zero Pseudomonas aeruginosa Azurin. The Journal of the American Chemical Society, 133:4865-73 (2011).
Warner, M., Lukose, V., Lee, K. H., Lopez, K., Sazinsky, M. H., and Crane, E.J. III.: Characterization of an NADH-Dependent Persulfide Reductase from Shewanella loihica PV-4: Implications for the Mechanism of Sulfur Respiration via FAD-Dependent Enzymes. Biochemistry, 50:194-206 (2011).